This simulation provides students with a highly customizable HPLC simulator that can allow them to collect realistic data for many compounds under a wide range of conditions.
The simulation of important concepts in HPLC is accomplished through the use of provided Excel macros. The exercise is written with explicit instructions that can be used as an at-home laboratory exercise or as supporting material for lectures on chromatograpic theory. Macros are included for isocratic, linear and step gradient, and non-ideal behaviors (Langmuir, adsorption at…