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Fluorometric Determination of Riboflavin in Energy Drinks

This experiment was designed as an at-home lab experiment where students use a portable fluorescence spectrometer to quantitate riboflavin in energy drinks. This experiment can be adapted for a range of educational levels, depending on the background information given. This work is adapted from the work of others (see below).

The attached file provides general instructions, suggested materials, experiment setup, and analysis. This information can be used to guide students in an at-home or in-lab project, or for faculty to quickly produce an experiment for students.

Photograph of Riboflavin-Containing Samples Under UV-light

References & Additional Resources

  1. “Quantification of Riboflavin in Energy Drinks” https://www.stellarnet.us/wp-content/uploads/StellarNet-Exp3_Fluor_Riboflavin.pdf (Accessed July 12, 2020)
  2. “Monitoring Vitamin B2 in Energy Drinks” January 31, 2013. https://www.vernier.com/vernier-ideas/monitoring-vitamin-b2-in-energy-drinks/ (Accessed July 12, 2020)

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